castration resistant prostate tumor cell lines pc3  (DSMZ)

Journal: Scientific Reports

Article Title: Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression

doi: 10.1038/s41598-024-72408-w

Figure Lengend Snippet: Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.

Article Snippet: Androgen-dependent 22RV1, LNCAP, VCAP, C4-2 and androgen-independent DU145and PC3 human tumor prostate cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Over Expression, Western Blot

Journal: Biochemical and biophysical research communications

Article Title: Ser815 Phosphorylation stabilizes the androgen receptor homodimer and stimulates ER-stress induced cell death.

doi: 10.1016/j.bbrc.2022.11.083

Figure Lengend Snippet: Fig. 1. AR S815D disturbs nuclear translocation and transactivation of AR WT upon DHT treatment. [A] PC-3 cells co-transfected with GFP-tagged AR WT and FLAG-tagged AR S815A or S815D and treated with 10 nM DHT for 1 h, from which cytosolic (Cyto.) and nuclear (Nuc.) fractions were prepared for subsequent Western blot assays. a-tubulin and Histone H1 were used as markers for cytosolic and nucleic fractions, respectively. [B] Reporter assay was performed using FLAG-tagged AR WT expressing with or without FLAG- tagged AR S815D into PC-3 cells. Cells were treated with vehicle (0.1% DMSO: 0 nM) or 10 nM DHT for 24 h. Firefly luciferase activities were normalized to renilla luciferase activities. Data represent means ± S.D. (n ¼ 3). *P < 0.05, **P < 0.01, ***P < 0.001 (Sidak's multiple comparison test).

Article Snippet: COS-1 cells (ATCC, Rockville, MD), human prostate tumor PC3 cells (ATCC), and human hepatocarcinoma Huh-7 cells were cultured in D-MEM media supplemented with 10% (v/v) heatinactivated fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin.

Techniques: Translocation Assay, Transfection, Western Blot, Reporter Assay, Expressing, Luciferase, Comparison

Journal: Biochemical and biophysical research communications

Article Title: Ser815 Phosphorylation stabilizes the androgen receptor homodimer and stimulates ER-stress induced cell death.

doi: 10.1016/j.bbrc.2022.11.083

Figure Lengend Snippet: Fig. 4. ER stress-induced cell death was accelerated by AR S815D mutation with DHT treatment. [A] PC-3 cells transfected with FLAG-tagged AR WT or mutant expressing vector or mock vector were treated with 10 nM DHT, followed by cell viability determination. Data represent means ± S.D. (n ¼ 3) [B and C] PC-3 cells transfected with FLAG-tagged AR S815A or S815D expression vector were pretreated with 10 nM DHT or vehicle for 24 h. Subsequently, cells were co-treated with 10 nM DHT or vehicle with brefeldin A, thapsigargin or vehicle for 24 h and cell viability was determined. Data represent means ± S.D. (n ¼ 3) *P < 0.05, **P < 0.01, ***P < 0.001 (Sidak's multiple comparison test).

Article Snippet: COS-1 cells (ATCC, Rockville, MD), human prostate tumor PC3 cells (ATCC), and human hepatocarcinoma Huh-7 cells were cultured in D-MEM media supplemented with 10% (v/v) heatinactivated fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin.

Techniques: Mutagenesis, Transfection, Expressing, Plasmid Preparation, Comparison

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: Dose–response analysis of sensitive and drug-resistant prostate cancer cells. Growth of cabazitaxel-sensitive versus cabazitaxel-resistant cells has not been evaluated on PC3 cells. Error bars indicate standard deviation (SD). * indicates significant difference to the corresponding control, n = 5.

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Standard Deviation, Control

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: Influence of amygdalin on the growth of parental, cabazitaxel- and docetaxel-resistant PC3, DU145, and LNCaP cell lines. Cell count is related to the 24 h value set to 100%. Error bars indicate standard deviation (SD), n = 5. * indicates significant difference to the corresponding non-treated control.

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Cell Counting, Standard Deviation, Control

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: ( A ): BrdU uptake in sensitive and drug-resistant PC3, DU145, and LNCaP cells following amygdalin exposure for 24, 48, and 72 h. Values are given in percentage and are related to untreated controls, which were set to 100%. ( B ): Number of PC3, DU145, and LNCaP clones (drug-sensitive, cisplatin-resistant, and gemcitabine-resistant) exposed to amygdalin (+A), compared to the nontreated controls (−A). Error bars indicate standard deviation (SD), n = 3. * indicates significant difference to untreated controls. n.d.: not done.

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Clone Assay, Standard Deviation

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: Cell cycle distribution in drug-sensitive and drug-resistant PC3, DU145, and LNCaP cells following amygdalin exposure (+A) for 24 (all cell lines) and 72 h (LNCaP cells). Controls (−A) remained untreated. One representative of three separate experiments is shown (n = 3).

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques:

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: Up: Western blot of cell cycle and mTOR-related proteins from drug-sensitive and drug-resistant PC3, DU145, and LNCaP cells. Tumor cells received either amygdalin (+A) or cell culture medium alone (−A) for 24 h. β-actin served as the internal control. One representative from three separate experiments. n.d.: not done. Down: The ratio of protein intensity/β-actin intensity expressed as a percentage of the controls, set to 100%. Error bars indicate standard deviation, n = 3. * indicates significant difference to controls. n.d.: not done. Original blots see .

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Western Blot, Cell Culture, Control, Standard Deviation

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: Adhesion of drug-sensitive and drug-resistant PC3 ( A ), DU145 ( B ), and LNCaP cells ( C ) to immobilized collagen, fibronectin, or matrigel. Tumor cells were either treated with amygdalin for 24 h (+A) or remained untreated (−A). ( D ) Effect of amygdalin (+A) on chemotactic migration of PC3 and DU145 cells. Controls were without amygdalin (−A). Bars indicate standard deviation (SD). * indicates significant difference to the corresponding control. n = 5. n.d.: not done.

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Migration, Standard Deviation, Control

Journal: Cancers

Article Title: Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells

doi: 10.3390/cancers14133111

Figure Lengend Snippet: Up: Western blot of cytoskeletal proteins and integrin β1 from drug-sensitive and drug-resistant PC3, DU145, and LNCaP cells. Tumor cells received either amygdalin (+A) or cell culture medium alone (−A) for 24 h. β-actin served as the internal control. One representative from three separate experiments. Down: The ratio of protein intensity/β-actin intensity expressed as a percentage of the controls, set to 100%. Error bars indicate standard deviation, n = 3. * indicates significant difference to controls. Original blots see .

Article Snippet: Human, castration-resistant prostate tumor cell lines PC3 and DU145 and castration-sensitive LNCaP cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Western Blot, Cell Culture, Control, Standard Deviation